Molecular Detection of Bacillus cereus in Milk by Polymerase Chain Reaction

It is a valuable and good food source with the ingredients it contains in milk, but if hygienic conditions are not ensured during milking, storage processing, diseases related to milk dairy products likely occur. More than 90% of these bacterial origin. B. cereus, found Bacillaceae family, significant contamination due presence psychrotrophic strains. In recent years, has been reported that contaminated cereus have adverse effects on human health, toxic substances formed products. 
 This study, aimed molecularly identify bacteria species cause such as poisoning loss quality milk. this DNA Bacillus one microorganisms raw was isolated gene regions were determined at molecular level by PCR method using specific primers cereus. Six for nheA, nheB, nheC, hblA, hblC, hblD 16S rRNA used detection samples. At end detected 8 13 revealed can be quickly reliably identification primers.